Success Story

October 5, 2017

CBC Junior Investigator, Sadie Wignall, NU, sheds light on female reproduction and infertility

Sadie Wignall (NU), was awarded a CBC Junior Investigator Award in 2010 when she joined the Department of Biochemistry, Molecular Biology and Cell Biology (renamed the Department of Molecular Biosciences) as an Assistant Professor. Sadie also received a 2016 CBC Catalyst Award. Her lab uses C.elegans as an experimental model to study spindle formation and chromosome segregation during the asymmetric divisions of oocyte meiosis. Two of her recent publications are featured below. Congratulations, Sadie!


New insights into leading cause of miscarriage, birth defects discovered

Studies begin to uncover why embryos sometimes lack correct amount of genetic material

A typical spindle (top, left) physically separates chromosomes during cell division. The bottom right image is a spindle in which two proteins were knocked out during research, causing the spindle to collapse into a messy round ball. (Credit: Northwestern University)

EVANSTON – Two recent Northwestern University studies shed new light on the mystery of the leading cause of birth defects and miscarriage, laying the foundation for further research in an understudied but crucially important field of genetic study.

The studies look at what happens during the process that produces egg cells (oocytes), which later become embryos when they are fertilized. Ten to 25 percent of human embryos contain the wrong number of chromosomes because the egg cell has not divided properly, which is a problem unique to egg cells.

These mistakes are the leading cause of miscarriages and birth defects such as Down syndrome, and the incidence of these errors rises dramatically as women age (see graph below). Understanding why egg cells are more prone to this division error is critical, given that women are increasingly choosing to start families at later ages.

The first study, published in the Journal of Cell Biology in March, revealed that oocytes use an innovative strategy to detect and prevent errors during cell division, while the second study, published Sept. 26 in PLOS Genetics, identified new proteins essential for the cell-division process and discovered that a back-up protein kicks in when the division is failing to help ensure the embryo is receiving the correct number of chromosomes.

“Taken together, these two studies have revealed to us how vastly different egg cells are from every other type of cell, which could shed important new light on why the reproductive process can be so error prone,” said senior author Sadie Wignall (right), assistant professor of molecular biosciences at Northwestern’s Weinberg College of Arts and Sciences. “Solving this mystery would be a first step to prolonging a woman’s fertile years.”

Wignall researches a structure called the spindle, an elaborate football-shaped structure that physically separates the chromosomes during cell division. In most cells, structures called centrosomes help to organize the spindle, ensuring that it can precisely separate chromosomes to send the correct number of chromosomes to each newly divided cell. Spindles in egg cells, however, lack centrosomes. This “acentrosomal” process is highly understudied compared to other types of cell division, leading to important unanswered questions about why it is much more prone to error when dividing.

In the study published in September, Wignall and her team discovered that in the absence of centrosomes, two proteins – KLP-15 and KLP-16 – were essential for dividing the cells. The researchers knocked out these two proteins to find that instead of forming the normal football-shaped spindle, the spindle structure collapsed into a messy round ball. Much to their surprise, despite this early defect, a back-up protein then jumped in and helped separate the chromosomes to the two ends of the cell.

“We were surprised to find that this protein came to the rescue and worked as a backup to properly organize the spindle,” Wignall said.

The question remains why 10 to 25 percent of embryos still end up not being viable if there is this backup process in place in oocytes. One theory, Wignall said, is that this backup protein changes or depletes as women age.

“While these basic cell mechanisms might be difficult to grasp, they directly impact female reproduction and infertility,” Wignall said. “My lab focuses on this with the hope that one day, our research can help people experiencing fertility issues at in vitro fertilization clinics.”

Wignall performs her research on oocytes using small worms called C. elegans since they are a powerful research organism for genetic studies. However, her lab is also building on these findings to perform parallel studies in mice in collaboration with Teresa Woodruff, a reproductive scientist and director of the Women’s Health Research Institute at Northwestern University Feinberg School of Medicine. The next step will be to study these mechanisms in human oocytes.


Amanda C. Davis-Roca, a graduate student in Wignall’s lab, was the first author on the study published in March, “Caenorhabditis elegans oocytes detect meiotic errors in the absence of canonical end-on kinetochore attachments.” Timothy J. Mullen, another graduate student in Wignall’s lab, was first author on the study published in September, “Interplay between microtubule bundling and sorting factors ensures acentriolar spindle stability during C. elegans oocyte meiosis.”


Source:

Adapted (with modifications) from Northwestern News, originally posted on October 02, 2017 by Kristin Samuelson.


Citations:

Davis-Roca AC, Muscat CC, Wignall SM. Caenorhabditis elegans oocytes detect meiotic errors in the absence of canonical end-on kinetochore attachments. J Cell Biol. 2017 May 1;216(5):1243-1253. (PubMed)

Mullen TJ, Wignall SM. Interplay between microtubule bundling and sorting factors ensures acentriolar spindle stability during C. elegans oocyte meiosis. PLoS Genet. 2017 Sep 14;13(9):e1006986. eCollection 2017 Sep. (PubMed)


See more:

CBC Junior Investigator Award (2010):
Jun Cheng (UIC), Alex Ruthenburg (UChicago), Daniel Dombeck (NU) and Sadie Wignall (NU)
▸ Four New Faculty Receive CBC Junior Investigator Awards