Cbc funded facilities
Advanced Imaging Core (AIC) -- a part of the Chicago Center for Systems Biology -- is currently employing a confocal imaging system coupled to a microfluidics platform to measure spatiotemporal expression of fluorescence tagged transcription factors in live embryos. For more information, please, click here.
Location: Chicago Center for Systems Biology, (UChicago campus), 900 East 57th Street, Chicago, IL 60637
The BAC-Recombineering Core at the Chicago Center for Systems Biology can provide investigators affiliated with CBC schools the capability to dynamically image fluorescently tagged proteins in model organisms and measure spatiotemporal expression of protein of interest. Such tagged proteins can be used further to purify their associated partners, and to identify their chromatin binding sites. Center Scientists employed and affiliated with the BAC-Recombineering Core can design and conduct experimental protocols and provide pre-experimental consultation to investigators who seek to incorporate this advanced transgenic technology into their research programs. For more information, please, click here.
Location: Chicago Center for Systems Biology, (UChicago campus), 900 East 57th Street, Chicago, IL 60637
Center for Advanced Molecular Imaging (CAMI)
The Center for Advanced Molecular Imaging is an imaging facility of unprecedented scope. It provides researchers with access to a wide variety of existing imaging modalities as well as new imaging technologies developed within the center. The facility is focused on providing multi-modal analysis of biological systems from the single cell to the whole animal.
State of the art cellular and molecular imaging services and training include the following modalities:
- Magnetic Resonance Imaging
- Optical Imaging
- Fluorescence and Bioluminescence Imaging
- Ultrasound Imaging (planned for future acquisition)
- Endoscopy (planned for future acquisition)
- Positron Emission Tomography (planned for future acquisition)
- Computed Tomography (planned for future acquisition)
- Single Photon Emission Computed Tomography (planned for future acquisition)
For more information, please, click here.
Location: Chemistry of Life Processes Institute, (NU-Evanston campus), 2170 Campus Dr., Evanston, IL 60208-2850
Cellular Screening Center (CSC)
The goal of the Institute for Genomics & Systems Biology (IGSB) is to accelerate the transition of basic discoveries in genome science into practical benefits for society. The Institute's Cellular Screening Center (CSC) houses a state-of-the art high throughput cellular screening facility. Support from the Chicago Biomedical Consortium and The University of Chicago Biological Sciences Division enabled the creation of the CSC. The CSC facilitates drug discovery and development by defining the complex genetics underlying disease, isolating chemical compounds that affect specific cellular activities, and developing potential therapeutics to target disease pathways. To learn more about IGSB's Cellular Screening Center (CSC) click here.
Location: Chicago Center for Systems Biology, (UChicago campus), 900 East 57th Street, Chicago, IL 60637
Chicago Tri-Institutional Center for Chemical Methods and Library Development (CTCMLD)
The mission of the Chicago Tri-Institutional Center for Chemical Methods and Library Development (CTCMLD) is to address the fundamental challenges of modern high-throughput organic synthesis and to provide major advances that will significantly facilitate the development of small-molecule libraries for broad biological screening. Support from the CBC has strengthened the core infrastructure of the CTCMLD that contains three integral components, the Library Production Core located on the University of Chicago campus, the Hit-to-Lead Development Resource (HLR) located at Northwestern University and the Computational Cheminformatics Core at the University of Illinois at Chicago. CTCMLD offers a fully automated system for production of high-quality chemical libraries, cheminiformatics, analysis of biogenicity of lead compounds and the ability to further optimize their efficacy and pharmacological properties. Contact information regarding submission of collaborative proposals is located on CTCMLD website.
Location: Library Production Core, (UChicago campus), 5735 S. Ellis Avenue , Chicago, IL 60637
Hit-to-Lead Development Resource, (NU campus), 633 Clark Street, Crown Center Room 2-502, Evanston, IL 60208
Computational Cheminformatics Core, (UIC campus), 1737 W. Polk Street, Chicago, IL 60612
The Computation Core at the Chicago Center for Systems Biology (CCSB) is led by Dr. Robert Grossman, Senior CCSB investigator & Director of the National Center for Data Mining (NCDM) at the University of Illinois at Chicago (UIC). The Core relays on standard bioinformatics tools as well as specialized tools such as:
Cistrack: Cistrack is a repository for transcriptional network studies.
Flynet: Flynet provides an integrated view of Drosophila melanogaster transcription regulation and makes genome wide in vivo protein-DNA interactions data available to the scientific community as a whole.
The Chicago Utility for Biological Sciences (CUBioS): The CBC/CUBioS project is an open-source cloud-based bioinformatics platform. Cistrack is CUBioS instance. CUBioS is available from Source Forge and can be customized to support a variety of bioinformatics applications.
For more information, please, click here.
Location: Chicago Center for Systems Biology, (UChicago campus), 900 East 57th Street, Chicago, IL 60637
A fully equipped atomic resolution analytical electron microscope with a high-brightness cold cathode field emission gun operated at 200kV. With its many analytical instruments, including STEM, GIF, and EDS, this unit forms the centerpiece of the TEM section of the Electron Probe Instrumentation Center (EPIC). EPIC is part of the NUANCE Center (Atomic - and Nanoscale Characterization Experimental Center) at Northwestern University.
For more information about Hitachi HD-2300A STEM, please, click here.
Location: NUANCE, (NU-Evanston campus), 2220 Campus Drive #2036, Evanston, IL 60208-3108
Mass spectrometry Metabolomics and Proteomics Facility (MMPF)
Mass spectrometry Metabolomics and Proteomics Facility (MMPF; formerly known as The Proteomics and Informatics Services Facility or PISF) is located on the University of Illinois at Chicago (UIC) campus. Through the facility, investigators affiliated with CBC member institutions have access to a number of mass spectrometers optimized for use in proteomics studies. Please visit the facility's website to learn more.
Location: Mass spectrometry Metabolomics and Proteomics Facility (MMPF), (UIC campus), University of Illinois at Chicago, 835 S. Wolcott Ave., MSB Room E-125, Chicago, IL 60612
Proteomics Center of Excellence (PCE)
The Proteomics Center of Excellence (PCE) is focused on the qualitative and quantitative detection of as many cellular components as possible at the protein level. The center is located on the Northwestern University-Evanston Campus and offers:
Top Down Proteomics
The Proteomics Center houses the best technology for “Top Down” Proteomics, which will push the limits for analysis of whole protein molecules. In Top Down proteomics, proteins are analyzed without digestion to get a handle on the dynamics of a whole protein.
Precision Bottom Up Proteomics
The center will implement known technologies for “Bottom Up”, but emphasize the philosophy of ‘precision proteomics,’ which uses high performance mass spectrometry to deliver protein identifications with very high confidence and in a gene-specific fashion. Quantitation of protein expression and confident assignment of post-translational modifications will also be possible in a highly collaborative framework.
Histone PTM Analysis
With the triple-quadrupole mass spectrometer, the center can provide quantitative site-specific information about methylations and acetylations on histones.
For more information, please, click here.
Location: Proteomic Center of Excellence (PCE), (NU-Evanston campus), Silverman Hall B550, 2170 Campus Dr., Evanston, IL 60208