Spatiotemporal Control of Protein Function: Ligand Activation of TEV Protease

Type of Award: Catalyst
Date Awarded: February 2016
Award End Date: January 2018
Amount Awarded: $ 200,000.00
PI(s): Howard Shuman, UChicago; Heather Pinkett, NU;

Abstract: Protein complexes carry out essentially all activities of living cells. Although remarkable progress has been made in determining the three dimensional structures of individual proteins, the detailed structures of multi-protein complexes are much more difficult to unravel. Even more challenging is to find out which parts of complexes interact with one another while carrying out their function. The proposed work will develop a simple method to precisely cleave protein complexes with a "protease" that can be targeted to specific sites in protein complexes. The main goal of the proposed work is to develop new forms of the protease that can be turned on by the presence of a small molecule that stabilizes special mutant forms of the protease. This will provide a new method to study protein structure and function.