Development of a 3D Snapshot Holographic Microscope for Volumetric Live Cell Imaging

Type of Award: Catalyst
Date Awarded: August 2014
Award End Date: July 2016
Amount Awarded: $ 199,633.00
PI(s): Norbert Scherer, PhD, UChicago; Oliver Cossairt, PhD, NU;

Abstract: We propose to develop a 3D Snapshot Holographic Microscope (3D-SHM) capable of imaging whole live cells in all 3 dimensions in a single "snapshot." The instrument combines independent developments in the Cossairt and Scherer/Jureller labs. This will enable imaging motion (dynamics) of cellular processes by brightfield, darkfield, and fluorescence microscopy modalities. A specific goal is developing greater understanding of intracellular transport and secretion of insulin granules in living cells. However, other biological and non-biological processes and systems can be studied with the 3D-SHM.