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Proteomics Facilities | |
| Location: | Argonne National Laboratory 9700 South Cass Avenue Building 202 Argonne, IL 60439 |
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| Contact: | csgiometti@anl.gov | |
Two-dimensional Gel Electrophoresis
 Original site of the Iso-Dalt system for 2DE, first described in 1979 by Norman Anderson and Leigh Anderson, the Biosciences Division at Argonne National Laboratory houses instrumentation capable of running 2DE gels in sets of twenty with a capacity of approximately 60 gels per week (http://proteomeweb.anl.gov/methods/index.asp). Gels are stained with Coomassie Brilliant Blue, silver nitrate, or ProQ (for phosophoproteins), digitized, and analyzed for quantitative differences using the Progenesis software package (Nonlinear USA). Oracle databases are used to organize samples, associated gels and data (http://gelbank.anl.gov/). |
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Mass Spectrometry
A QStar XL quadrupole time-of-flight mass spectrometer interfaced with an LC Packing nanoflow HPLC system is used for tandem mass spectrometry analysis of tryptic peptides generated from in-gel or in-solution digestions. Protein identifications are obtained using Mascot to search in-house protein sequence databases. All publicly available, as well as some proprietary, sequence databases are available on local servers.
A novel method for managing multiple annotations for a given protein sequence allows use of multiple protein sequence databases for searches (http://bioinformatics.anl.gov/SEGUID). Oracle databases are used to manage the sample identifiers, mass spectra, and protein identifications.
